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Ibis and Thoth
Ibis and Thoth
Sources : Wikipedia and Shoarns.

Growing colony of E. coli cells.
Growing colony of E. coli cells.
Source : Stewart et al. (2003), PLoS Biol., 3(2) :e45.

Network of global regulators involved in the carbon starvation response of E. coli.
Network of global regulators involved in the carbon starvation response of E. coli.
Source : Monteiro et al. (2008), Bioinformatics, 24(16) :i227-332.

Left : Example of a genetic regulatory network of two genes (a and b), each coding for a regulatory protein (A and B). Protein B inhibits the expression of gene a, while protein A inhibits the expression of gene b and its own gene. Right : PL model with step functions describing this genetic regulatory network.
Left : Example of a genetic regulatory network of two genes (a and b), each coding for a regulatory protein (A and B). Protein B inhibits the expression of gene a, while protein A inhibits the expression of gene b and its own gene. Right : PL model with step functions describing this genetic regulatory network.
Source : Batt et al. (2008), Automatica, 44(4):982-989.

Glucose concentration controls a bacterial billboard.A microplate containing a minimal medium (with
glucose and acetate) is filmed during 36 hours. Wells
contain E. coli bacteria which are
transformed with a reporter plasmid containing the
luciferase operon (luxCDABE) under control of the
acs promoter . This promoter is positively regulated
by the CRP-cAMP complex. When bacteria have
metabolized all the glucose, the cAMP concentration increases
quickly and activates the global regulator CRP which
turns on the transcription of the luciferase operon
producing the light. The glucose concentration
increases from left to right on the microplate ; so its
consumption takes more time when going up the
gradient and the letters appears one after the other .
The luciferase protein needs reductive power
(FMNH2) to produce light. At the end, when acetate
has been depleted, there is no more carbon source
in the wells. As a consequence, the reductive power
falls and the
Glucose concentration controls a bacterial billboard.A microplate containing a minimal medium (with glucose and acetate) is filmed during 36 hours. Wells contain E. coli bacteria which are transformed with a reporter plasmid containing the luciferase operon (luxCDABE) under control of the acs promoter . This promoter is positively regulated by the CRP-cAMP complex. When bacteria have metabolized all the glucose, the cAMP concentration increases quickly and activates the global regulator CRP which turns on the transcription of the luciferase operon producing the light. The glucose concentration increases from left to right on the microplate ; so its consumption takes more time when going up the gradient and the letters appears one after the other . The luciferase protein needs reductive power (FMNH2) to produce light. At the end, when acetate has been depleted, there is no more carbon source in the wells. As a consequence, the reductive power falls and the "bacterial billboard" switches off.
Source : Guillaume Baptist (Université Joseph Fourier).

Network of global regulators involved in the carbon starvation response of E. coli.
Network of global regulators involved in the carbon starvation response of E. coli.
Source : Monteiro et al. (2008), Bioinformatics, 24(16) :i227-332.

Left : Example of a genetic regulatory network of two genes (a and b), each coding for a regulatory protein (A and B). Protein B inhibits the expression of gene a, while protein A inhibits the expression of gene b and its own gene. Right : PL model with step functions describing this genetic regulatory network.
Left : Example of a genetic regulatory network of two genes (a and b), each coding for a regulatory protein (A and B). Protein B inhibits the expression of gene a, while protein A inhibits the expression of gene b and its own gene. Right : PL model with step functions describing this genetic regulatory network.
Source : Batt et al. (2008), Automatica, 44(4):982-989.

Glucose concentration controls a bacterial billboard.A microplate containing a minimal medium (with glucose and acetate) is filmed during 36 hours. Wells contain E. coli bacteria which are transformed with a reporter plasmid containing the luciferase operon (luxCDABE) under control of the acs promoter . This promoter is positively regulated by the CRP-cAMP complex. When bacteria have metabolized all the glucose, the cAMP concentration increases quickly and activates the global regulator CRP which turns on the transcription of the luciferase operon producing the light. The glucose concentration increases from left to right on the microplate, so its consumption takes more time when going up the gradient and the letters appears one after the other . The luciferase protein needs reductive power (FMNH2) to produce light. At the end, when acetate has been depleted, there is no more carbon source in the wells. As a consequence, the reductive power falls and the
Glucose concentration controls a bacterial billboard.A microplate containing a minimal medium (with glucose and acetate) is filmed during 36 hours. Wells contain E. coli bacteria which are transformed with a reporter plasmid containing the luciferase operon (luxCDABE) under control of the acs promoter . This promoter is positively regulated by the CRP-cAMP complex. When bacteria have metabolized all the glucose, the cAMP concentration increases quickly and activates the global regulator CRP which turns on the transcription of the luciferase operon producing the light. The glucose concentration increases from left to right on the microplate, so its consumption takes more time when going up the gradient and the letters appears one after the other . The luciferase protein needs reductive power (FMNH2) to produce light. At the end, when acetate has been depleted, there is no more carbon source in the wells. As a consequence, the reductive power falls and the "bacterial billboard" switches off.
Source : Guillaume Baptist.