WellInverter : web application for analyzing reporter gene data
WellInverter is a web application developed in the Ibis group. It implements linear inversion methods for the reconstruction of gene expression profiles from fluorescent or luminescent reporter gene data.
As input, WellInverter reads the primary data file produced by a 96-well microplate reader (Tecan), containing time-series measurements of the absorbance (optical density) as well as the fluorescence and luminescence levels in each well (if available). Various modules exist to analyze the data, in particular for detecting outliers, subtracting background, estimating growth rates, promoter activities and protein concentrations, visualizing expression profiles, synchronizing replicate profiles, etc.
Access to WellInverter
The WellInverter server can be accessed at the following address :
Please contact Hidde de Jong for obtaining a user account or consult the Supplementary Information of the paper submitted for publication for access information.
More information on how to use WellInverter can be found in the tutorial.
The Python library WellFARE, implementing the linear inversion methods on which WellInverter is based, is separately available under an LGPL license at the following address :
Developers of WellInverter
WellInverter has been developed by Michel Page, Valentin Zulkower, with help from Hidde de Jong, Johannes Geiselmann, and Delphine Ropers.
V. Zulkower, M. Page, D. Ropers, J. Geiselmann, H. de Jong, Robust reconstruction of gene expression profiles using linear inversion. Submitted for publication.