Background Definition Window

The background definition window helps you defining which wells can be used to correct for background levels in absorbance, luminescence, and fluorescence data in other wells.

Defining the status of a well

The window shows:

• A left listbox with the type of measurements considered (typically absorbance, luminescence, and fluorescence)
• The microplate with the wells that can be selected as backgrounds or for which backgrounds can be selected

To assign a status to a well or a group of wells, you simply have to click on it or draw a rectangle with the mouse. When the button is released, a context menu shows up that allows you to assign to these wells a background well, define the well as a background well, etc. There can be no ambiguity on the status: if for example some wells are defined to be corrected by means of background well C3, the latter well is automatically defined as a background.

On the bottom side, a small legend explains the meaning of the colors:

• A red well, labeled BG, is a background well for the current type of measurement
• A white well is a well that does not need to be corrected for this type of measurement
• A green well is corrected based on the background well it is labeled with.

On the lower left side, a preview of the data allows you to check the contents of a well.

Propagation of changes in background

when you change the definition of background wells, the measurements depending on this background are automatically corrected with the new background.

Note: Redefining backgrounds currently sets the associated parameters (e.g., the fit parameter) to their default values.