Lexicon

Absorbance

Absorbance is one of the types of measurement. It is a measurement of the size of the bacterial population. See also: Fluorescence, Luminescence.

Corrected Data

The corrected data are obtained by removing outliers, adjusting smoothing parameters, and possibly applying a background correction. See also: Original Data.

Detection Limit

In the promoter activity and concentration windows, data are taken into account only when above the detection limit.

Flag

Values are flagged by the analysis device when some experimental conditions are not fulfilled. For example, a T means that the temperature requirements were not met when the measurement was made.

Fluorescence

Fluorescence is one of the types of measurement. It is a measure of the accumulation of fluorescent protein in the bacterial population. See also: Absorbance, Luminescence, RFU.

Growth Rate

Growth rate is the derivative of the natural logarithm of the absorbance. It is one of the parameters used for background correction. See also: Lag Time, BackgroundCorrection?, BackgroundCorrectionWindow.

Lag Time

The lag time is the lag before the growth of the bacterial population starts. It is one of the parameters used for background correction. See also: Growth Rate, BackgroundCorrection?, BackgroundCorrectionWindow.

Luminescence

Luminescence is one of the types of measurement. It is a measure of the accumulation of the luminescent protein (luciferase) in the bacterial population. See also: Absorbance, Fluorescence, RLU.

Microplate

A microplate contains 96 wells, in a 12*8 array. Each is named after its position on the plate, ranging from A1 to H12.

Original Data

Original Data are the readings of the microplate reader without any corrections. See also: Corrected Data.

Outlier

Outliers are erratic values in the data set. See also: OutlierDetectionWindow.

RFU

Relative Fluorescence Unit See Fluorescence

RLU

Relative Luminescence Unit See Luminescence

Smoothing parameter

Parameter for the spline fit, ranging between 0 and 1. The lower the number, the smoother the curve. See also: FitWindow.

Type of measurement

A type of measurement is one of Absorbance, Fluorescence, and Luminescence.

Well

There are 96 wells per microplate. Each well contains a bacterial population for which absorbance, fluorescence, and luminescence can be measured.